Testing for Cough
What causes coughing?
Coughing can be caused by many different diseases. A few examples include:
- inflammation or irritation of the respiratory passages due to allergies, tumors, or inhaled foreign material such as food particles or plant fragments
- infection of the respiratory system due to bacteria, viruses, fungi, or parasites
- pneumonia – infection of the lungs
- tracheal collapse – caused by a weak trachea (windpipe) that flattens on itself when the pet inhales (breathes in) rather than staying open like a rigid pipe
- heart disease, especially congestive heart failure
How do we determine the cause of my pet’s cough?
The search for answers starts with a complete history and physical examination. A pet’s “history” is the information you give the veterinarian about your pet’s illness. In a coughing pet this would include details such as how long the cough has been present, possible contact with other pets, and whether the cough is triggered by any particular situation. History can be very helpful; for example, a dog that develops a cough after staying in a boarding kennel may have a respiratory infection called kennel cough; a toy breed dog that coughs only when it’s excited may have a collapsing trachea; a coughing cat with a history of being outdoors may have feline heartworm disease.
A thorough physical examination involves looking at all parts of the body, examining the mouth and throat, and listening to the heart and lungs with a stethoscope. This examination may provide clues about the cause of cough. For example, a pet with an irregular heart beat or heart murmur may have heart disease, the presence of loud raspy sounds in the lung may indicate underlying fungal pneumonia, a dog who coughs easily when the throat area is touched may have tracheitis or a collapsing trachea.
"...additional diagnostic tests may be needed and
your veterinarian may recommend doing screening tests..."
History and physical examination provide important information, but additional diagnostic tests may be needed and your veterinarian may recommend doing screening tests. These are a series of simple tests that provide information about the overall health of the pet and may provide further clues about the underlying problem.
What screening tests would be recommended?
In a coughing pet, the recommended screening tests could include: complete blood count (CBC), serum biochemistry profile, urinalysis, and parasite tests. Further testing may be recommended depending on the results of the initial screening tests.
What can these screening tests tell us?
(a) Complete Blood Count: This is a simple blood test that provides information about the different cell types in blood. These include red blood cells, which carry oxygen to the tissues, white blood cells, which fight infection and respond to inflammation, and platelets, which help the blood to clot. The CBC provides details about the number, size and shape of the various cells types, and identifies the presence of abnormal cells in circulation. (See article Complete Blood Count).
In a coughing pet, a CBC might show:
- anemia (decreases in the number of red blood cells and the amount of hemoglobin). This could be due to bleeding in the respiratory system, or could be a sign of longstanding respiratory inflammation.
- polycythemia (increases in the number of red blood cells and the amount of hemoglobin): This is seen with longstanding lung problems and represents the body’s attempt to increase the amount of oxygen the blood can carry to the tissues.
- increased numbers of white blood cells with increased number of neutrophils (a type of white blood cell): This often signals underlying infection.
- increased numbers of eosinophils: Eosinophils are a type of white blood cell associated with allergies and parasitic infections.
b) Serum biochemistry profile
Serum biochemistry profile refers to the chemical analysis of serum, which is the pale yellow liquid part of blood that remains after the cells and clotting factors have been removed. There are many substances in serum, including proteins, enzymes, fats, sugars, hormones, electrolytes etc. Measuring the levels of these substances provides information about the organs and tissues in the body, as well as the metabolic state of the animal. Changes and abnormalities found in the biochemistry profile can help to diagnose a variety of diseases and disorders. (See article Serum Biochemistry)
(c) Urinalysis is a simple test that analyzes the physical and chemical composition of urine. It measures how well the kidneys are working, identifies inflammation and infection in the urinary system, and helps to detect diabetes and other metabolic disturbances. Urinalysis is important for the proper interpretation of the serum biochemistry profile and should be done at the same time as blood testing. (See article Urinalysis)
... the serum biochemistry profile and urinalysis help to determine if a cough is part of a larger “systemic” illness…”
In a coughing pet, the serum biochemistry and urinalysis help to determine if a cough is part of a larger “systemic” illness. A systemic illness is a disorder that affects many organs and body systems at one time and can involve the lungs. If serum biochemistry and urinalysis are abnormal then systemic disease may be causing the cough, and further investigation of the underlying illness would be recommended. However, if the serum biochemistry and urinalysis are normal, then the cough likely involves only the respiratory system and further testing would investigate problems affecting only the lungs and airways.
(d) Parasite testing: In a coughing pet, both stool and blood should be tested for parasites.
1. Stool tests: Parasites that infect the lungs can be detected by examining a pet’s stool for parasite eggs or larvae (baby worms). The two most common stool tests are fecal flotation and fecal Baermann.
- Fecal flotation identifies the presence of parasite eggs in stool, while fecal Baermann detects the presence of larvae. Both tests require only a small amount of fresh stool. A simple version of the fecal flotation can be done in the veterinary clinic, but for best results, the stool sample should be sent to the laboratory where concentration techniques are used.
- Fecal Baermann is used specifically to find parasite larvae. It is a more a complicated test and samples are usually submitted to the laboratory for testing.
2. Blood test: Testing for Heartworm (Dirofilaria immitis) may be indicated if a pet lives in an area where heartworm infection is common or has visited an area where heartworm infection occurs. Heartworm disease is seen mostly in dogs but is seen in cats and both cats and dogs should be tested. Even a dog on a preventative program should be tested for heartworm infection if a cough develops. The test requires a simple blood sample.
What specific tests may be required if my pet’s cough just involves the respiratory system?
"There are many specific tests that might be recommended depending on the…history, physical examination, and screening tests."
There are many specific tests that might be recommended depending on the results of a pet’s history, physical examination and screening tests. A few examples would include:
- Imaging studies: X-rays and ultrasound of the chest would be recommended to look for evidence of heart disease, tumors, fluid accumulation in the chest, fungal infection, or parasites.
- Bronchoscopy allows the veterinarian to look directly down the trachea and respiratory passages to identify problems (see below).
- Transtracheal wash and bronchoalveolar lavage are techniques that collect material from the trachea and lungs for microscopic examination to look for causes of cough (see below).
- Sputum cytology: Sometimes a pet will cough up material and “spit” it on the floor. This material is called sputum and it can be examined microscopically for organisms, parasites, inflammation and cancer etc.
- Fine needle aspiration of the chest (thoracocentesis). This technique involves inserting a needle carefully through the body wall into the chest cavity and pulling out fluid that has built up there. This fluid is submitted to a veterinary referral laboratory for examination by a veterinary pathologist. This technique can identify the presence of infection, inflammation, fungal disease, parasites, and cancer.
- Fine needle biopsy. This is similar to fine needle aspiration, except that samples are collected from solid tissue such as the lung or a lung mass.
- Culture and sensitivity: if bacterial infection is suspected, culture and sensitivity is used to identify what types of bacteria are present and which antibiotic is best to treat the infection.
- Fungal serology: this is a blood test that helps to detect certain types of fungal infections.
- Testing for heart disease: This would include chest X-rays, cardiac echocardiogram, electrocardiogram, and possibly ProBNP blood testing.
When is a transtracheal wash indicated?
A transtracheal wash is indicated when a cough is due to a problem in the windpipe (trachea) or the large airways of the lung, called bronchi. A transtracheal wash involves flushing a small amount of sterile fluid into the trachea and then quickly suctioning or “re-aspirating” the fluid back into the syringe. The aim is to collect secretions, cellular material, and any infectious organisms such as bacteria, fungi, or parasites present in the trachea.
There are two ways of performing a transtracheal wash. The first method is done with mild sedation and local freezing of the throat area. The procedure involves making a small cut in the skin over the larynx or “voice box” and passing a special catheter (long tiny tube) through the skin and into the trachea. A syringe of sterile fluid is attached, and fluid is flushed into the trachea and then quickly re-aspirated back into the syringe.
The second method is done under general anesthetic with the placement of an endotracheal tube (a large bore tube that is routinely placed in the trachea during general anesthesia). A small catheter is threaded down the endotracheal tube into the trachea and a syringe is used to flush sterile fluid into the trachea and re-aspirated, as described for the first method.
Bronchoscopy involves passing a small flexible fiberoptic endoscope down the windpipe to look directly at the lining of trachea and bronchi. If there are signs of inflammation, bleeding or other disease, small biopsies can be collected for later microscopic examination. Bronchoscopy can also identify the presence of certain parasites that live in the trachea, and can detect malformations such as collapsing trachea. A general anesthetic is required for bronchoscopy.
"... bronchoalveolar lavage is used to investigate disease deep in the lung tissue"
Bronchoalveolar lavage is used to collect samples from deep in the lung. The technique involves passing an endoscope down the trachea and into a small airway in the lung. Then, similar to a transtracheal wash, a small amountof sterile fluid is flushed into the airway and then quickly re-aspirated. The collected material is sent to a veterinary pathologist for examination. A general anesthetic is required for bronchoalveolar lavage.
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